The Comparison of Optimum Conditions for Lead (Pb) Analysis Method using DITHIZONE and EDTA Complex in Seaweeds (Eucheuma spinosum)

Abstract

The comparison of optimum conditions for lead (Pb) analytical method using dithizone and EDTA complexes in Eucheuma Spinosum (Eucheuma Sp.) was measured using a standard addition method, where the standard solution of Pb2+ was added to the sample to reduce noise. Additionally, UV-Vis spectrophotometer was used with dithizone and EDTA complexes in Eucheuma Spinosum, where Pb2+ ions in both complexes were measured. Both complexes were first developed to determine the optimal pH that would measure Pb2+ level in Eucheuma Spinosum. The most favorable pH level for such measurement was pH 8, at which pH activity was most stable, and the most favorable complex was dithizone complex, with a regression value closer to one than that of EDTA complex.