Utilization of rice wastewater as an alternative medium for growth and quality of Shiitake mushrooms (Lentinula edodes)

Abstract

Multiplication of Shiitake (Lentinula edodes) as a pure breeding commonly uses Potato Dextrose Agar (PDA) as the medium. Further, the cost of its basic material, which relatively expensive needs an alternative medium to produce pure brood stocks for Shiitake and to reduce the cost of producing pure brood stocks such as utilizing rice wastewater. Rice wastewater known to have composition as complete as PDA. The research aimed to find the utilization of rice wastewater as Shiitake mycelial growth medium and determine the best concentration of rice wastewater medium on the growth rate and quality of Shiitake mycelium. The research used an experimental with descriptive analysis method consisting of 11 treatments of rice wastewater concentration i.e. 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 100%, and PDA as the control, all treatments were replicated three times. The results showed that various concentrations of rice wastewater affected on the growth rate of Shiitake mycelium. The concentration of 100% rice wastewater produced the best growth and quality of mycelium compared to other treatments. Therefore, the rice wastewater can be used as an alternative medium to multiply pure broodstocks of Shiitake mushrooms.