Structure of Proteus mirabilis 027 O‐specific polysaccharide containing amino acids and phosphoethanolamine

Abstract

The following structure of the repeating unit of the O‐specific polysaccharide of Proteus mirahilis 027, containing amides of d‐glucuronic and n‐galacturonic acids with l‐lysine and l‐alanine, respectively, N‐acetyl‐d‐glucosamine and phosphoethanolamine, has been determined: (Formula Presented.) The main methods used for structural analysis of the polysaccharide were selective solvolysis with anhydrous hydrogen fluoride and Smith degradation, as well as NMR spectroscopy and mass spectrometry. The immunodominant role of the lateral N‐acetyl‐d‐glucosamine and phosphoethanolamine in manifesting the serological specificity of P. mirubilis 027 has been established. Copyright © 1989, Wiley Blackwell. All rights reserved