An optimal establishment of an acute hyperglycemia zebrafish model

Abstract

Recently, zebrafish has shown the potential to become an important in vivo model for diabetes-related research. In this study, we performed a validation study for the establishment of optimal hyperglycemia involving the following factors: alloxan concentrations of 100, 200, 300, and 400 mg/100 ml; exposure times of 30, 60, 120, and 180 min to an alloxan solution and water glucose solution; and water glucose solution concentrations of 1, 2, and 3%. The results have shown that exposure to water glucose solution following alloxan treatment might increase blood glucose level in zebrafish in a dose- and time-dependent manner. The appropriate hyperglycemia zebrafish model was induced in 300 mg/100 ml alloxan solution for 30 min, 1% water glucose solution for 30 min, and water for 1 h. We suggest that our zebrafish model could be an alternative hyperglycemia animal model in the future.