STUDY of CHITINASE and CHITINOLYTIC ACTIVITY of LACTOBACILLUS STRAINS

Abstract

The increasing consumer demand for less processed and more natural food products-while improving those products' quality, safety, and shelf-life-has raised the necessity of chemical preservative replacement. Biopreservation refers to extended storage life and enhanced safety of foods using the natural microflora and (or) their antibacterial products. Chitinolytic enzymes are of biotechnological interest since their substrate, chitin. is a major structural component of the cell wall of fungi, which are the mam cause of the spoilage of food and raw plant material. Among the several organisms, many bacteria produce chitinolytic enzymes, however, this behaviour is not general. The chitinase activity of the lactic acid bacteria is scarcely known and studied. The aim of the present study was to select Lactobacillus strains that have genes encoding chitinase. furthermore, to detect expressed enzymes and to characterise their chitinase activity. Taking into consideration the importance of chitin-bindig proteins (CBPs) in the chitinase activity; CBPs were also examined. Five Lactobacillus strains out of 43 strains from 12 different species were selected by their chitinase coding gene. The presence of the chitinase and chitin-bidrng protein production were confirmed, however, no chitinolytic activity has been identified.