Regional and generalized changes in cytosolic free calcium in monocytes during phagocytosis

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Abstract

We measured and visualized cytosolic free calcium ([Ca2+](i)) in individual human peripheral blood monocytes during phagocytosis by using the fluorescent indicator fura-2. Monocytes exhibit a rapid rise in [Ca2+](i) from a basal level of 75 ± 11 nM to a peak level of 676 ± 78 nM (means ± standard errors of the means; P < 0.001) by 34 ± 5 s after contact with opsonized zymosan particles, and a thin rim of high [Ca2+](i) was observed surrounding the ingested particle.

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Kim, E., Enelow, R. I., Sullivan, G. W., & Mandell, G. L. (1992). Regional and generalized changes in cytosolic free calcium in monocytes during phagocytosis. Infection and Immunity. https://doi.org/10.1128/iai.60.3.1244-1248.1992

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