Steroidal Saponins from the Underground Parts of Hosta longipes and their Inhibitory Activity on Tumor Promoter-Induced Phospholipid Metabolism

Abstract

Phytochemical study on the underground parts of Hosta longipes gave six new steroidal saponins together with a known one. The structures of the new compounds were determined by detailed analysis of their 1H- and 13C-NMR spectra including two-dimensional NMR spectroscopy, acid-catalyzed hydrolysis followed by chemical correlation, and by comparison with spectral data of known compounds. The isolated saponins and their aglycones were examined for inhibitory activity on 12-o-tetradecanoylphorbol-13-acetate (TPA)-stimulated 32P-incorporation into phospholipids of HeLa cells to identify new antitumor-promoter compounds. © 1995, The Pharmaceutical Society of Japan. All rights reserved.