Goiter and hypothyroidism in two siblings due to impaired Ca+2/NAD(P)H-dependent H2O2-generating activity

Abstract

We report herein the study of two siblings (DESM and DSM) with hypothyroidism, goiter, and positive perchlorate discharge tests (50% and 70%) in a family (M) with no history of consanguinity. Thyroid gland histology showed a predominance of hyperactive follicles, with high epithelial cells and variable colloid content. Thyroid peroxidase iodide oxidation (DESM, 1034; DSM, 1064 U/g protein) and albumin iodination (DESM, 16; DSM, 8 nmol I/mg protein) activities were within the normal range. Tg content was normal in both glands compared with that in diffuse toxic goiter (DESM, 28; DSM, 17; diffuse toxic goiter, 19 mg/g tissue), and Tg could be normally iodinated by thyroid peroxidase in vitro (DESM, 3.4; DSM, 4.3; diffuse toxic goiter, 6.3 nmol I/mg Tg). Thyroid cytochrome c reductase activities in these goiters were higher than that in paranodular tissues (DESM, 473; DSM, 567; paranodular tissues, 78 nmol NADP +/h/mg protein). However, thyroid NADPH oxidase activities were very low both in the particulate 3,000 × g (DESM, 4.8; DSM, 44; paranodular tissues, 224 nmol H2O2/h/mg protein) and in the particulate 100,000 × g fractions (DESM, 40; DSM, 47; paranodular tissues, 200 nmol H2O2/h/mg protein). Thus, a decreased Ca2+/NAD(P)H-dependent H2O2 generation is the probable cause of the organification defect in these goiters.