A beginner’s guide to ChIP-seq analysis

Abstract

Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a method to identify DNA-binding regions of specific proteins or histone modifications throughout the genome. It uses antibodies against these proteins and next-generation sequencing (NGS) technology to obtain the DNA sequences of the binding regions. Like other methods that use NGS, ChIP-seq method requires basic data analysis skills. In this beginner’s guide, we explain the basic steps of the ChIP-seq experiment and its data analysis steps. The goal of this article is to help readers to improve their understanding of ChIP-seq experiments, data analysis, and the insights from data analysis results.