Carbapenem Resistant Gene of Pseudomonas Aeruginosa

Abstract

Objective: Determining the frequency of MBLs classifying VIM, IMP and NDM in P. aeruginosa isolates. Methods: The isolates of P. aeruginosa were isolated from sputum, blood, pus, fluid from patients (non-duplicate samples) in different clinical departments. They had been identified and performed antibiotic susceptibility testing by using Vitek 2 according to CLSI 2017 standards at Cho Ray Hospital from October 2017 to March 2018. The isolates were subcultured, picked colonies, extracted DNA and perform real-time PCR with MDR-MBL kit (VIM, IMP, NDM) of Sacace (Italy) to detect the MBL class VIM, IMP and NDM. Results: There were 64 isolates of P. aeruginosa resistant to carbapenem (either or both Imipenem and Meropenem) were carried out real-time PCR to detect MBL (VIM, IMP, NDM). Among them, 29 (45.3%) isolates had IMP-type gene, 7 (10.9%) isolates had NDM-type gene, 3 (4.7%) isolates had VIM-type gene, 3 (4.7%) isolates had both IMPtype and NDM-type genes, 22 (34.4%) isolates were negative for the above 3 genes. 10 carbapenem-susceptible isolates were performed real-time PCR to detect MBL (IMPtype, NDM-type, VIM-type). Among them, 2 (20%) isolates had IMP-type gene, 8 (80%) isolates were negative for 3 genes. Conclusion: The results of this study revealved a noticeably high prevalance of MBL- producing P. aeruginosa isolates at our hospital. MBL genes among isolates which reported as sensitive to carbapenem was acting as reservoir of such resistance genes with potential risk for the silent spread of these genes in hospitals and community. So, early recognition of MBL-producing isolates, establishing efficacy infection control measures and an appropriate protocol for antibicrobial therapy based on the laboratory data are necessary to prevent the dissemination of these resistance.