Bovine vitreous gel can reacvate replicave senescence of human dermal fibroblast

Abstract

The most influenal factor in the poor healing of chronic ulcers is replicave senescence of fibroblasts that are unresponsive to TGF-β1 smulaon. Cellular replicave senescence can be induced by culvang normal human dermal fibroblasts (HDFs) in a serum-starved medium. In addion, increasing microenviroment mechanical forces by hyaluronic acid can ameliorate the TGF-β1 signaling of these senescent cells. One of natural resources of hyaluronic acid is bovine vitreous gel. In order to evaluate the effect of bovine-vitreous gel on replicave senescence of fibroblasts, we used various levels of bovine vitreous gel diluted in Dulbecco’s modified Eagle’s medium to smulate cellular acvies of serum-starved HDFs. Those cellular acvies were compared to the control media, standardized hyaluronic acid, and to normal HDFs. Our results show that replicave senescence of HDFs treated with 50% bovine vitreous gel exhibited a significantly higher proliferaon index, migraon rate, and collagen deposion than those cultured in control media, and they displayed an equal level of cellular acvity with the HDFs exposed only to standardized hyaluronic acid. We concluded that bovine vitreous gel can be used to smulate replicave senescence of HDFs and therefore a potenal candidate material to smulate healing of chronic ulcers.