Protective effects of salidroside and dexamethasone against E.coli-induced inflammatory response on endometrial epithelium cells in Yaks

Abstract

Endometritis is an inflammation of the uterus, which can reduce the reproductive performance in yaks. Salidroside is a natural monomer of Rhodiolarosea, it has anti-inflammatory, antioxidative and immune regulation properties. We conduct this study to investigate the effect of salidroside and dexamethasone on E. coli-induced inflammatory response on endometrial epithelium cells in yaks. For this purpose, the endometrial epithelial cells were cultured and dived into four groups: control group, E.coli group, salidroside group and dexamethasone group. The antioxidant response, cytokine assays and immunofluorescence staining analysis were performed. The results showed that the intracellular activities of SOD were decreased, while MDA contents were increased significantly in E.coli-induced inflammation group. Whereas, the MDA contents decreased and SOD increased in salidroside and dexamethasone supplement groups. Moreover, E. coli results in significantly increase in cytokine production. The TNF-α, IL-1α, IL-6 and IL-10 levels in the cells treated with salidroside and dexamethasone were decreased significantly compared to those of the E.coli group. E.coli-induced inflammatory cells showed an increase in the level of TNF-α protein compared to control group. The salidroside administration reduced the TNF-α expression significantly just like dexamethasone. In conclusion, salidroside and dexamethasone play important role in regulating E.coli-induced inflammatory response in endometrial epithelium cells. Furthermore, salidroside treatment is encouraged for the control of endometritis, which may target to reduce the incidence of endometritis.