Assessment of the effectiveness and biocompatibility of hydrogel patch incorporated with drug on denture induced traumatic ulcers

Abstract

To fabricate and compare the effectiveness of a hydrogel incorporated with Benzocaine and Aloe Vera for wound healing. An in-vitro study was conducted with three different formulations of hydrogel, Group 1 (control group), benzocaine group (Group2) and aloe Vera group (Group3) of 1x1cm dimension. The physical and chemical parameters were observed to assess the efficiency of the hydrogel for wound healing in -vitro. The physical examination all the benzocaine hydrogel patch samples appeared smooth, colourless and whereas; Aloe Vera incorporated hydrogel was granular had odour similar to fenugreek seeds. The scratch test revealed mean migration rate of 10.69?μm/hr in group1 while group 2 and group 3 showed 16.25 and 13.19 respectively. The MTT assay showed cell cytotoxicity rate in UMR106 cells with IC50 values inhibition of 90.34?μg/ml, 96.03μg/ml, and 81.99μg/ml in control group, group1 and group 2 respectively. The Aloe Vera patches were the most biocompatible among the 3 groups and, Benzocaine hydrogel showed the fastest cell migration rate. The evaluation of physical properties concluded that both Aloe Vera and Benzocaine hydrogel can be used for drug delivery.