Correction: ADAMTS5 is a critical regulator of virus-specific t cell immunity (PLoS Biology (2016) 14:11 (e1002580) DOI: 10.1371/journal.pbio.1002580)

Abstract

An influenza-specific CD8+ T cell peptide encoded within the nucleoprotein (NP) was incorrectly referred to as NP366-372 throughout the article. The correct designation should be NP366-374. The peptide sequence ASNENMETM stated throughout the 'Methods' section should also be attributed to NP366-374. The authors have provided corrected versions of Fig 3, Fig 4, Fig 5, Fig 8, S10 Fig and S11 Fig. The relevant figure legends have been amended to reflect this change and are presented below. Supporting information S10 Fig. Influenza virus infection of Vcan+/hdf mice. Lung tissue and MLNs were removed from influenza virus infection C57.BL/6 and Vcan+/hdf mice and processed to generate single cell suspensions at day 10 p.i. for analysis of influenza-specific immunity. (A) Total CD8+ T cell numbers were determined at day 10 p.i. in the lung. (B) Influenza-specific DbNP366-374+ CD8+ and DbPA224-233+ CD8+ tetramer positive T cells in the lung were enumerated at day 10 p.i. CD8+ T cell functionality was measured using ICS. (C) Influenza specific DbNP366-374+IFNγ+CD8+ and DbPA224-233+IFNγ+CD8+ T cell responses were characterised in the lung at day 10 p.i. (D) Total CD8+ T cell numbers were determined at day 10 p.i. from pooled MLN samples. (E) Influenza-specific DbNP366-374+ CD8+ and DbPA224-233+ CD8+ tetramer positive T cells in pooled MLN were enumerated at day 10 p.i. (F) CD8+ T cell functionality was measured using ICS to assess influenza-specific DbNP366-374+IFNγ+CD8+ and DbPA224-233+IFNγ+CD8+ T cell responses at day 10 p.i. The results are expressed as means ± SD or as pooled means (MLN data) and statistical significance (relative to C57.BL/6 mice) determined by a Student's t test (*p ≤ 0.05, ***p ≤ 0.005 relative to C57.BL/6, n = 5 representing three individual experiments). WT denotes C57.BL/6 mice. Underlying data are provided in S2 Data. (TIF) S11 Fig. Influenza infection of Adamts5-/- and WT littermate controls. Adamts5-/- and WT mice were infected i.n with X31 (H3N2) influenza virus and spleens, lungs, and MLNs removed from C57.BL/6 and Adamts5-/- mice days 7 p.i. Single cell suspensions were then analysed for influenza-specific immunity. (A) Weight loss was calculated over the time course of infection. Total CD4+ and CD8+ T cells were enumerated in the (B) spleen, (C) lung, and (D) MLN. Influenza-specific DbNP366-374+ CD8+ and DbPA224-233+ CD8+ tetramer positive T cell numbers were also characterised in the (B) spleen, (C) lung, and (D) MLN. Lung and spleen results are expressed as means ± SD or as pooled means (MLN data), and statistical significance (relative to C57.BL/6 mice) was determined by a Student's t test (*p ≤ 0.05, **p ≤ 0.01 relative to C57.BL/6 mice, n = 5 representing three individual experiments). WT denotes C57. BL/6 mice. Underlying data are provided in S2 Data. (TIF).