Fluorescent dyes for differential counts by flow cytometry. Does histochemistry tell us much more than cell geometry?

Abstract

A description is given in the form of a poem (74 couplets of 4 lines each, with the music written out - for all stanzas the same). Flow (?) cytometry, groups of the various verses being subdivided correctly into an introduction, the material and methods, the results and 2 couplets for acknowledgments. It is pure fun, to compare in this way different cytometric techniques, with illustrations on e.g. the partitioning of a leukocyte population into subpopulations using interactive compputer programs to isolate clusters seen on scatter grams. There is an illustration of the frequency distributions (histograms) of the flow cytometric parametrs of a population of about 1,600 leukocytes from a healthy subject, together with a simultation, by serial scatter grams, of the observed joint frequency distribution of 3 parameters for this population. One couplet follows here, just taken at random to give an impression of this article (sung, at the symposium on Automation and Cytochemistry, 28th Annual meeting, at Chicago, Ill. April 2, 1977: Some dyes are more specific than some other dyes, it is true, but more specific techniques may be harder ones to do. A basophil count done by IgE receptor sites would not give you much more information that you would get with Wright's. The text of this curious artcle gives references to 54 articles.