HPTLC method for simultaneous estimations of ramipril and losartan potassium in pharmaceutical dosage form: Development and validation considerations

Abstract

This paper describes validated high-performance thin-layer chromatography (HPTLC) methods for simultaneous estimation of ramipril (RAM) and losartan potassium (LOS) in pure powder and formulation. The HPTLC separation was achieved on an aluminum-backed layer of silica gel 60F254 using methanol: ethyl acetate: toluene: glacial acetic acid (1:9:1:0.2 v/v/v/v) as mobile phase. Quantification in HPTLC method was achieved with UV detection at 210 nm over the concentration range of 300 - 1300 ng/spot for RAM and 3000 - 13000 for LOS, respectively, with recovery of 98.93 - 99.73 and 98.96 - 100.11% for RAM and LOS, respectively. These methods are simple, specific, precise, sensitive and robust; they are applicable for the simultaneous determination of RAM and LOS in pure powder and formulation.