The Role Of OprD Gene In The Carbapenems Resistance In Pseudomonas Aeruginosa Isolated From Burns Infection In Al-Najaf Province

Abstract

Carbapenems-resistant Pseudomonas aeruginosa mutants are disseminating around the globe. 90%-100% of P. aeruginosa isolates became Carbapenems-resistant mutants in some countries like Thailand and Brazil respectively. Finding alternative therapeutic options instead of carbapenems is the global duty of researchers and institutions. The OprD gene mutation/s has the main role in carbapenems resistance appearance in P. aeruginosa due to the decrease in the outer membrane permeability for carbapenems. Aim: This study aims to assess the antimicrobial activity of carbapenems (imipenem, and meropenem) against Pseudomonas aeruginosa isolated from hospitalized burn patients in Al-Najaf/Iraq, and the role of OprD gene mutations in the resistance. Methods and materials: 76 samples were collected from hospitalized burn patients suffering from burn wounds infections, and the identity of each cultivated microbe was confirmed by VITEK compact system 2 and further confirmation for P. aeruginosa isolates via performing molecular identification by OprD gene detection, 10/12 carbapenems resistant isolates, 1/12 sensitive, and 1/12 intermediate resistant were submitted to OprD gene sequencing by Sanger method