The effect of microbial proteases on the activity of matrix metalloproteinases and oxidative stress indicators in wound tissue of rats with experimental diabetes mellitus

Abstract

Aim. To study the effect of microbial proteases on the activity of matrix metalloproteinases and oxidative stress indicators in wound tissue of rats with experimental diabetes mellitus (DM). Methods. Skin burns were induced in the animals without somatic pathology and with the background diabetes mellitus. DM was reproduced by a single injection of streptozotocin, 50 mg/kg. The multi-enzyme proteolytic composition Pronase (Sigma-Aldrich, USA), obtained from the culture fluid of Streptomiceus griseus, was applied to the wound. The content of malonic dialdehyde (MDA), as well as the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione (GSH) were measured on a spectrophotometer. The collagenolytic activity of matrix metalloproteinases (MMPs) of skin tissues was determined by enzyme-phoresis (gelatin zymography). Results. At the physiological wound healing, an application of the composition increased the activity of total MMPs in homogenate on days 3–14 of the healing process, which enhanced the formation of free radicals and the activity of antioxidant system to compensate tissue damage. Thus, the CAT, GSH and SOD levels were elevated on days 7, 14 and 21, respectively. In case of DM, an application of the composition enhanced the activity of MMPs on days 14–21, which improved the proteolytic degradation of extracellular matrix proteins in the state of excessive glycation and did not worsen oxidative homeo-stasis state in the wound. Conclusion. The use of exogenous proteases is appropriate to enhance proteolysis in tissues with predominance of glycated proteins in case of chronic hyperglycemia to ensure controlled proteolysis.