One-pot green synthesis and bioapplication of l-arginine-capped superparamagnetic fe 3o 4 nanoparticles

Abstract

Water-soluble l-arginine-capped Fe 3O 4 nanoparticles were synthesized using a one-pot and green method. Nontoxic, renewable and inexpensive reagents including FeCl 3, l-arginine, glycerol and water were chosen as raw materials. Fe 3O 4 nanoparticles show different dispersive states in acidic and alkaline solutions for the two distinct forms of surface binding l-arginine. Powder X-ray diffraction and X-ray photoelectron spectroscopy were used to identify the structure of Fe 3O 4 nanocrystals. The products behave like superparamagnetism at room temperature with saturation magnetization of 49.9 emu g -1 and negligible remanence or coercivity. In the presence of 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride, the anti-chloramphenicol monoclonal antibodies were connected to the l-arginine-capped magnetite nanoparticles. The as-prepared conjugates could be used in immunomagnetic assay. © 2009 to the authors.