Antimicrobial Activity of the Anthocyanins Isolated from Purple Field Corn (Zea mays L.) Cob against Candida spp

Abstract

The phytochemical compounds from purple field corn (Zea mays L.)cob (PFCC), mainly grown in Phitsanulok, Thailand, were extracted with acidified 80% methanol and the anthocyanins were isolated from the extract through column chromatography with acidified absolute methanol. The PFCC extract (PFCCE) showed total anthocyanins glucoside as 6022 mg/100 g by colorimetric method. Three kinds of anthocyanins were detected by HPLC-DAD at 500 nm. The antimicrobial activity of the anthocyanins from PFCC was then evaluated via macrobroth dilution method against Candida albicans ATCC 90028, two clinical isolates of Fluconazole susceptible species (C. albicans and C. tropicalis) and three clinical isolates of Fluconazole resistant species (C. glabrata, C. krusei, and C. parapsilosis). As a result, the anthocyanins exhibited a potent inhibitory activity against all the tested species with the MIC in range of 0.625 – 2.5 mg/mL. Moreover, fungicidal activity of the anthocyanins was also observed against clinical isolates of C. tropicalis and C. albicans with the MFC in range of 0.625 – 1.25 and 2.5 mg/mL, respectively