Effects of Ulva lactuca and Sargassum cinereum supplemented diets on haematological parameters and survival of Koi carp (Cyprinus carpio L.) against bacterial pathogen (Aeromonas species)

Abstract

The objective of the present study was to determine effects of Ulva lactuca and Sargassum cinereum supplemented diets on some selected blood parameters and survival of fry stage Koi carp (Cyprinus carpio Linnaeus, 1758). Fish were fed Ulva lactuca diet (T1), Sargassum cinereum diet (T2) and Control diet (C) for 14 weeks. Haematological parameters were measured at the end of the indoor experiment. Total erythrocyte count (TEC), total leucocyte count (TLC) and thrombocyte count were in the range of 1.44-1.49×106/mm3, 5.30-6.01×104/mm3 and 2.05-2.48×104/mm3 respectively. Thrombocyte count and TLC in T1 and T2 were significantly higher (p<0.05) than the control. A challenge-test was conducted to determine their survival against Aeromonas species. Two treatments (T1, T2) and the control (C) were included into experimental setup in triplicates and each treatment consisted with two groups; saline injected and bacteria injected groups. For each replicate eight healthy Koi carps were introduced. It was revealed that survivorship curves and median survival of seaweed supplemented treatments were significantly higher (p<0.05) from C. The highest survival percentage (25%) in the challenge-test was recorded in T2 at the censored point after 6 hours.