Crystallization of myosin subfragment 1

Abstract

Crystals of myosin subfragment 1 from avian skeletal muscle have been grown reproducibly. They diffract X-rays to at least 4.5-Å resolution. The subfragment 1 crystallizes in space group P212121 where a = 107 Å, b = 117 Å, and c = 278 Å. The cell dimensions and intensity distribution on X-ray diffraction photographs are consistent with two molecules in the crystallographic asymmetric unit. Electrophoretic analysis shows that the myosin subfragment 1 present in the crystals contains a 95-kilodalton heavy chain fragment and both the essential and regulatory light chains.