Use of electrophoretic light scattering for investigation the parameters of macromolecules

Abstract

We present a new method for investigation of the electrophoretic mobility and radius of macromolecules based on electrophoresis in the regime of total internal reflection. The theory of dynamic light scattering and its application for the analysis of data obtained by the technique of capillary electrophoresis in the regime of total internal reflection is considered. Experimental setup for simultaneously separation and registration of macromolecules under study was developed. The results of studies of suspensions with latex microspheres are discussed.