Nano for bio: Nanopore arrays for stable and functional lipid bilayer membranes (Mini Review)

Abstract

The usefulness of nanotechnology for biotechnological applications is frequently emphasized. The recent development for using nanostructured materials as supports for free-standing lipid bilayers is briefly reviewed. The authors then demonstrate that the stability of fragile free-standing lipid bilayers in nanopores is enhanced up to days depending on the surface chemistry, the lipid composition, and the diameter of the pores. The insertion of a pore forming protein into bilayers can be monitored over time as a stepwise decrease of membrane resistance. Since membrane proteins are major drug targets, such stable and functional proteo-bilayers integrated in microfluidics are the key components of in vitro devices for drug screening. This conference paper reviews the recent literature and provides preliminary results from own research.