Direct observation of single protein molecules in aqueous solution

Abstract

(Figure presented) Real-time visualisation and tracking: Fluorescent labeled protein molecules are tracked using high-speed imaging systems. The figure shows the fluorescence signal from single quantum dots integrated over 2.7 ms. Diffusion coefficients deduced from single-molecule traces are consistent with those determined independently by fluorescence correlated spectroscopy, demonstrating in differnt distances to the focal plane the effectiveness of the technique. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.