Alleviation of cisplatin-induced nephrotoxicity in albino rats by roots of Catunaregam uliginosa

Abstract

Objectives: To evaluate the nephroprotective effect of ethanolic extract of roots of Catunaregam ulginosa (EECU) in cisplatin-induced nephrotoxicity in albino rats. Methods: Ethanolic extract was prepared by hot extraction method and subjected to preliminary phytochemical studies. Based on acute toxicity studies, nephroprotector was screened at two dose levels, i.e., 200 mg/kg bd. Wt. and 400 mg/kg bd. Wt. in both curative and prophylactic regimens. Cisplatin at a dose of 5 mg/kg bd. Wt. was given intraperitoneally to induce nephrotoxicity. Nephroprotector activity was assessed by determining blood urea nitrogen, serum creatinine, urinary total protein (UTP), and creatinine clearance (Clcr). Antioxidant enzymes such as super oxide dismutase (SOD), catalase (CAT), glutathione reduced (GSH) activities, and lipid peroxidation (LPO) levels were determined in renal tissue. Histological studies had been carried per se. Results: Animals that received cisplatin exhibited a significant increase in serum marker levels, increased UTP excretion, and reduced Clcr. Further significant decrease in GSH, SOD, CAT, and increase in LPO levels was observed. EECU reversed the effects induced by cisplatin in a dose-dependent manner in both curative and prophylactic regimens. Histological studies substantiated the above results. Conclusion: The findings of the present study support the alleviation of cisplatin-induced nephrotoxicity by the EECU and thus validate its ethnomedicinal use.