Hispidospermidin, a novel phospholipase C inhibitor produced by chaetosphaeronema hispidulum (cda) moesz NR 7127. II. Isolation, characterization and structural elucidation

Abstract

Hispidospermidin (1) is a novel phospholipase C inhibitor produced by Chaetosphaeronema hispidulum (Cda) Moesz NR 7127. Its structure (C25H47N3O) has been elucidated as a cage compound with a trimethylspermidine side chain based on various NMR studies, including 1H-1H COSY, 13C-1H COSY, HOHAHA, HMBC, COLOC and long range J C-H resolved 2D spectroscopy. The absolute configuration of 1 has been elucidated by modified Mosher's method on the (R)-and (S)-MTPA amides of a derivative of 1. © 1994, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.