Integration and oligomerization of bax protein in lipid bilayers characterized by single molecule fluorescence study

Abstract

Background: The stoichiometry of Bax oligomers on mitochondria and the process of Bax pore formation remain unclear. Results: Bax oligomerization occurs only if tBid is present, and its membrane insertion highly depends on cardiolipin; Bax tends to form tetramer on membrane. Conclusion: Bax pore formation involves two independent steps: Bax oligomerization and membrane insertion. Significance: This study may help elucidate Bax pore formation.