Stable Isotopes in Human Nutrition—Laboratory Methods and Research Applications

Abstract

This book provides readers a brief glimpse into the manufacturing processes of the various stable isotopic tracers as well as a basic understanding of the principles, study designs, sample requirements, sample preparation techniques, instrumentation requirements and methods of calculation for several stable isotope methods commonly used in nutrition-related research. The book is written with the intention of generating excitement among new users and serves as a basic reference guide to experienced users. This is, therefore, appropriate for graduate-level curriculum in nutrition and for those at any level who wish to obtain an understanding of these methods. Introduction / W.W. Wong and S.A. Abrams -- Production of stable isotopes for nutrition-related research / W.W. Wong and S.A. Abrams -- The measurement of protein kinetics with stable isotope tracers / F. Jahoor -- Stable isotope studies of mineral metabolism : calcium, magnesium and iron / S. Abrams -- Evaluation of trace mineral status and bioavailability using stable isotopes (zinc, copper, selenium, molybdenum) / N.M. Lowe and C.S. Broome -- Energy utilization with doubly labelled water (²H₂¹⁸O) / W.W. Wong -- Body composition measurements with ²H and ¹⁸O isotope dilution / W.W. Wong -- Stable isotopes and gas chromatography/mass spectrometry in studies of glucose metabolism in children / A.L. Sunehag and M.W. Haymond -- Cholesterol and other lipid metabolism / R.E. Ostlund.