Distribution of nivalenol in milling fractions of severely Fusarium-infected Japanese soft winter wheat grains

Abstract

The fate of Fusarium mycotoxin nivalenol during milling of a Japanese wheat cultivar was investigated. Grain samples with two distinct nivalenol levels were test-milled to produce six flour fractions (Breaking flours: 1B, 2B, and 3B, Middling flours: 1M, 2M, and 3M) and two outer layer fractions (bran and shorts). Patent flour for human consumption was made from 1B, 1M, 2B, and 2M, while low-grade flour was made from 3B and 3M. These four samples; Patent flour, low-grade flour, bran and shorts were analyzed for the content of nivalenol by HPLC-UV. Two samples showed similar patterns of nivalenol distribution in milling fractions.