Improving accuracy of glucose oxidase procedure for glucose determinations on discrete analyzers

Abstract

We describe a rapid kinetic glucose oxidase (EC 1.1.3.4) procedure for quantifying glucose. Glucose oxidase concentration was reduced from the more usual 20 kU/L to 4 kU/L, and pH was reduced from 7.0 to 6.6. Potassium ferrocyanide (20 μmol/L) and ascorbate oxidase (1 kU/L) were incorporated in the procedure. The assay results vary linearly with glucose concentration from 0 to 50 mmol/L and are unaffected by bilirubin concentrations ≤600 μmol/L, hemoglobin ≤ 12 g/L, Intralipid® ≤4 g/L, urate ≤1 mmol/L, and ascorbate ≤2.0 mmol/L. The assay is readily adaptable to most open-system analyzers.