DOP08 Deep remission in paediatric Crohn’s disease is associated with increased abundance of dialister species and increased valerate

Abstract

S045 and invasive ability of E. coli between AIEC+ and AIEC− patients. The ERIC-PCR profiles of ileal E. coli showed that CD AIEC+ were for 78% of them colonised by not more than 2 clonal AIEC strains. In addition, AIEC were able to interact with CEACAM6 by binding D-mannose residues and to induce CEACAM6 expression in T84 cells (p = 0.0009 and p = 0.0185, vs. non-AIEC; respectively). This was also supported by adhesion inhibition test. Serum anti-E. coli level was higher for CD AIEC+ (vs. CD AIEC-). Ileal CEACAM6 level were positively correlated with abundance of ileal associated E. coli in AIEC+ patients (r = 0.4000; p = 0.0362) and with salivary CEACAM6 level (r = 0.4690; p < 0.0001). The non-invasive biomarker 'serum anti-E.coli/salivary CEACAM6' index was higher for CD AIEC+ (p = 0.0174; vs. CD AIEC-). A cutoff value < 1.34 × 10 −6 eliminated the presence of ileal AIEC with a high negative predictive value (90% CI95% [69%-95%]). Conclusion: Our study reported that identification of faecal AIEC cannot replace identification of AIEC from ileal biopsies, most of AIEC infection are mono or bi-clonal (≤ 2 strains) and that non-invasive biomarker such as 'serum anti-E.coli/salivary CEACAM6' index could be helpful to screen CD patients for AIEC infection. Background: Faecal microbiota transplantation (FMT) provides replacement of pathogenic bacteria with more favourable microbi-omes in recipients with dysbiosis. The aim of the present study was to prospectively investigate the efficacy of FMT by assessing the clinical and endoscopic response in patients with ulcerative col-itis (UC) who had failed anti-inflammatory, immunosuppressive and TNF-α inhibitors (Infliximab, Adalimumab, Vedolizumab) therapy. Methods: In this prospective and uncontrolled study, 116 patients with UC were enrolled. All medications except mesalazine were discontinued 1 week before FMT. Colonoscopy was performed both before and after FMT. To assess the efficacy of FMT, Mayo scores were calculated at weeks 0, 24 and 48. A total of 400-600 ml of extracted fresh faecal suspension was administered into the 20 to 30 cm of terminal ileum of recipients. Results: After 4.5 years of FMT experience with 116 patients, who have completed their 6 months after 236 procedures of FMT for treatment of UC, 42 of the 116 patients showed full clinical response (100% clinical + laboratory + endoscopically full response) (per-protocol analysis 37.1%), (intention-to-treat analysis 36.2%) at Week 48 and 33 of the 116 patients achieved clinical and endoscopic remission (laboratory 70%, clinically and endoscopically 50-75% improvement) (per-protocol analysis 29.2%), (intention-to-treat analysis 28.4%) at Week 48.