Candida albicans’in moleküler tespiti ile sütçü ineklerde Candida mastitisi

Abstract

This study focused on determining the prevalence of Candida species involved in dairy cattle mastitis with molecular detection of Candida albicans. A total of 150 milk samples were collected from dairy cattle showing clinical mastitis. Isolation and identification of organisms through phenotypical and physiological criteria on different media were performed as well as molecular identification of C. albicans. Fourty one isolates of Candida species were recovered with a prevalence of 27.3%. C. albicans was the dominating species (29.3%). Out of 12 strains phenotypically identified as C. albicans, 8 were confirmed by PCR using species specific primer for the 26S rRNA gene of C. albicans. A specific virulence determinant Phospholipase B1 gene was detected in all molecularly identified C. albicans isolates. This study has clearly shown the prevalence of Candida mastitis and providing a new attractive diagnostic molecular tool for mycotic mastitis caused by C. albicans.