Location of the proanthocyanidins in the barley grain

Abstract

A vanillin-HCl staining has been used to locate the proanthocyanidins in mature barley grains. These flavanoids were found to be concentrated in the seed coat (testa) just outside the aleurone layer in the 7 varieties with yellow coloured grains investigated. The seed coats of 10 proanthocyanidin-free barley mutants belonging to 4 different gene loci did not stain with vanillin-HCl. © 1984 Carlsberg Laboratory.