Absorption of thermoxidized fats. I. Reproducibility and accuracy of the previous analytical techniques used for the evaluation of non-absorbed lipids

Abstract

Reproducibility of the techniques used for isolation and separation of non-absorbed lipids present in feces from rats fed with diets containing heated oils are defined. Highly reproducible results have been found for total lipids, nonpolar fatty acid compositions. Results obtaines from extracted diets demonstrate both the absence of significant alteration and the complete extraction of the fat when compared to those obtained directly from the oils. Non-absorbed lipids from diets containing thermoxidized oils are characterized by high proportions of unsaponifiable compounds and polar fatty acids.