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Enumeration of Legionella CFU by colony hybridization using specific DNA probes

Applied and Environmental Microbiology (2002) 68(12) 6466-6470
DOI: 10.1128/AEM.68.12.6466-6470.2002
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Abstract

Oligonucleotide probes and colony hybridization (CH) were applied to enumerate organisms of the genus Legionella in cooling tower water. The CH counts indicated almost the same results as CFU counts in cultivated samples derived from the water. It was concluded that it is possible to substitute the CH procedure for the conventional one.

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Satoh, W., Nakata, M., Yamamoto, H., Ezaki, T., & Hiramatsu, K. (2002). Enumeration of Legionella CFU by colony hybridization using specific DNA probes. Applied and Environmental Microbiology, 68(12), 6466–6470. https://doi.org/10.1128/AEM.68.12.6466-6470.2002

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