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SNP genotyping by DNA photoligation: Application to SNP detection of genes from food crops

Science and Technology of Advanced Materials (2009) 10(3)
DOI: 10.1088/1468-6996/10/3/034603
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Abstract

We describe a simple and inexpensive single-nucleotide polymorphism (SNP) typing method, using DNA photoligation with 5-carboxyvinyl-2′-deoxyuridine and two fluorophores. This SNP-typing method facilitates qualitative determination of genes from indica and japonica rice, and showed a high degree of single nucleotide specificity up to 10 000. This method can be used in the SNP typing of actual genomic DNA samples from food crops. © 2009 National Institute for Materials Science.

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Yoshimura, Y., Ohtake, T., Okada, H., Ami, T., Tsukaguchi, T., & Fujimoto, K. (2009). SNP genotyping by DNA photoligation: Application to SNP detection of genes from food crops. Science and Technology of Advanced Materials, 10(3). https://doi.org/10.1088/1468-6996/10/3/034603

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