Liquid Chromatographic resolution - Development and characteristics of the chiral stationary phases consist of polysaccharide derivatives and application to large-scale separation -

Abstract

The importance of optically active compounds for pharmaceutical use has increased. The high-performance liquid chromatographic resolution on chiral stationary phases is useful for large-scale preparation of optically active compounds and estimations of their separating costs as well as determination of optical purity. Chiral stationary phases consisting of polysaccharide derivatives were developed by adsorbing them onto silica gel based on the findings obtained from cellulose triacetate. The characteristics of cellulosic carbamates (OC, OF, OG, OD) and amylosic carbamates (AS, AD) as well as cellulosic esters (OA, OB, OJ, OK) in chiral recognition are considered here along with resolved compounds. Optimization of the separating conditions was performed while considering differences among acidic, neutral and basic analytes. Furthermore, we introduced the principle and applications on the Simulated Moving Bed process to produce optically active compounds on the industrial scale.