Detection of extended-spectrum β-lactamase enzymes (ESBLs) produced by Escherichia coli urinary pathogens at Assiut University Hospital

Abstract

This study was conducted to evaluate the rate of urinary tract infection at Assiut University Hospitals, and to determine the prevalence of extended-spectrum β-lactamase enzymes producing community- and hospital-acquired E. coli uropathogens at Urology Department in Assiut University Hospitals. Also, to perform the antimicrobial sensitivity testing for E. coli isolates to extended-spectrum cephalosporins according to CLSI. Phenotypic confirmatory tests (combined disc method, double disc diffusion method and ESBL-E-Test) were performed to test E. coli isolates for ESBL production and it was concluded that 30 (39.47%) of community isolates and 42 (70%) of nosocomial isolates were ESBLs producers.