Evaluation of the Efficacy of Crude Extracts of Salix subserrata and Silene macroselen for the treatment of rabies in Ethiopia

Abstract

A study was conducted between November, 2007 and April, 2008 to evaluate the anti-rabies activity of the leaf of Salix subserrata and root of Silene macroselen which are traditionally used for the treatment of rabies in Bereh-Aleltu Woreda, North Shewa, Central Ethiopia. The study involved oral administration of crude extracts of Salix subserrata and Silene macroselen to Pasteur Virus (PV) strain rabies virus infected mice which were assigned into three treatment and one negative control groups. Chloroform, methanol 80% and aqueous extracts of the study plants were prepared using maceration technique. Statistical analysis was performed by one-way analysis of variance tests coupled to least significant difference to compare result between treatment and positive control groups. Of the 6 crude extracts, chloroform and methanol 80% of Silene macroselen and chloroform and aqueous of Salix subserrata were found to increase the survival time of mice significantly (P Keywords: Anti-rabies, Ethno-pharmaceutical, Mice model, PV strain, Salix subserrata, Silene macroselen