Phytochemical analysis, antioxidant and anti-inflammatory activity of Leaf extracts of Merremia emarginata

Abstract

The plant Merremia emarginata was extracted with ethanol and from the ethanolic extract, ethyl acetate fraction was prepared and to the above products preliminary phytochemical studies was performed and it shows the presence of flavonoids, steroids, terpenoids, tannins, carbohydrates, amino acid and glycosides. To the ethanolic extract of Merremia emarginata leaves and ethyl acetate fraction TLC was performed in various mobile phases and detected by using UV chamber, concentrated sulphuric acid and iodine chamber. The Rf values were noted. It was found that the extract and fractions has both fluorescent and non-fluorescent compounds. The column chromatography was performed for the Ethyl acetate fractions in normal phase by increasing the polarity. The fractions were collected and conducted the TLC. So, at the fraction of (Hexane: Ethyl Acetate (6:4)), single spot was observed. The EEME and HEAFME were screened for their antiinflammatory and antioxidant activities. Keywords: Merremia emarginata, Column chromatography, Anti-inflammatory and Antioxidant activity