Antimicrobial susceptibility testing of Arcobacter butzleri and Arcobacter cryaerophilus isolated from buffalo milk with subclinical mastitis: A different approach

Abstract

The aim of to our study was the minimum inhibitory concentrations (MICs) of nine antimicrobials in current use and three potentially new alternatives against Arcobacter spp. isolated from dairy buffalos with subclinical mastitis, and to evaluate these parameters instead of pharmacokinetic parameters. The Arcobacter spp. isolates were isolated from milk samples collected from dairy buffalos with subclinical mastitis. The susceptibility of Arcobacterspp. strains to antimicrobials were performed according to the guidelines by the NCCLS. The MIC value of vancomycin, erythromycin and tetracycline were not determined, and MIC value of ceftiofur, spiramycin and gentamicin have showed wide variations for isolated strains. However, cefquinome, tylosin, enrofloxacin and florfenicol were determined the best-performing agents against these strains. Antibiotics show concentration and time dependent killing, and studies have demonstrated the AUC/MIC, Cmax/MIC and t MIC ratios to be the best diviner of antibacterial effect. In the present study, based on the MIC values determined for selected antimicrobial agents, and pharmacokinetic parameters, amoxicillin, ceftiofur, cefquinome, enrofloxacin and florfenicol may be appropriate for the treatment of mastitis infections caused by susceptible Arcobacter spp. in buffalos