Effects of Rice–Fish Coculture on Greenhouse Gas Emissions: A Case Study in Terraced Paddy Fields of Qingtian, China

Abstract

Rice–fish coculture, a traditional integrated agriculture–aquaculture system, has been recognized as a “Globally Important Agricultural Heritage System” due to its ecological and socio-economic benefits. However, the impact of rice–fish coculture on greenhouse gas emissions remains controversial. This study investigated the effects of rice–fish coculture on methane (CH4) and nitrous oxide (N2O) emissions in the Qingtian rice–fish system, a 1200-year-old terraced paddy field system in Zhejiang Province, China. A field experiment with two treatments, rice–fish coculture (RF) and rice monoculture (RM), was conducted to examine the relationships between fish activities, water and soil properties, microbial communities, and greenhouse gas fluxes. Results showed that the RF system had significantly higher CH4 emissions, particularly during the early rice growth stage, compared to the RM system. This increase was attributed to the lower dissolved oxygen levels and higher methanogen abundance in the RF system, likely driven by the grazing, “muddying”, and burrowing activities of fish. In contrast, no significant differences in N2O emissions were observed between the two systems. Redundancy analysis revealed that water variables contributed more to the variation in greenhouse gas emissions than soil variables. Microbial community analysis indicated that the RF system supported a more diverse microbial community involved in methane cycling processes. These findings provide new insights into the complex interactions between fish activities, environmental factors, and microbial communities in regulating greenhouse gas emissions from rice–fish coculture systems. The results suggest that optimizing water management strategies and exploring the potential of microbial community manipulation could help mitigate greenhouse gas emissions while maintaining the ecological and socio-economic benefits of these traditional integrated agriculture–aquaculture systems.