We have developed a method for real-time monitoring of the cell responses to cytotoxicants using Fourier transform infrared spectroscopy with the multiple internal reflection (MIR-FTIR) geometry. To prevent cell damages induced by measurement environments, we have constructed specialized chambers, in which temperature was maintained at (37±0.5) °C and humidified air containing 5% C O2 was supplied. We monitored cell death induced by cytotoxic surfactant Tween20 using MIR-FTIR spectroscopy. It was found that cell death can be monitored by the absorption intensity of amide II band. This result suggests that our method has a potential to be applied for real-time cytotoxicity assay. © 2007 American Institute of Physics.
CITATION STYLE
Yamaguchi, R. T., Hirano-Iwata, A., Kimura, Y., Niwano, M., Miyamoto, K. I., Isoda, H., & Miyazaki, H. (2007). Real-time monitoring of cell death by surface infrared spectroscopy. Applied Physics Letters, 91(20). https://doi.org/10.1063/1.2813013
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