Micropropagação de um híbrido de Eucalyptus (Eucalyptus benthamii x Eucalyptus dunnii)

Abstract

This study was designed to micropropagate E. benthamii x E. dunnii, by testing chlorine concentrations for explant asepsis, the optimal concentrations of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) for bud proliferation, and the ratio between BAP and gibberellic acid (GA 3) in two nutrient media for shoot elongation. Nodal segments from H12, H19 and H20 clones were disinfected with 0.5, 1.0, 1.5 and 2.0% (v v -1) of chlorine. Explants were grown on 1/2MS medium supplemented with BAP (0, 0.25, 0.50, 0.75 and 1.00 mg L -1) and NAA (0, 0.025, 0.050, 0.075 and 0.100 mg L -1) for bud production. They were elongated on MS and 1/2MS media supplemented with BAP (0, 0.05 and 0.10 mg L -1) and GA 3 (0, 0.1, 0.2 and 0.3 mg L -1). The 0.50 mg L -1 BAP and 0.050 mg L -1 NAA combination was optimal for bud proliferation for H12 and H20. GA 3 concentrations of 0.10 and 0.20 mg L -1 combined with 0.10 mg L -1 BAP on 1/2MS resulted in the longest shoots, for H12 and H20, respectively. Regardless of clone, the rooting rate was low, with an average of 12.0% and 14.4% of plants having roots for in vitro and ex vitro conditions, respectively.