Identification of an IgE-binding region in soybean acidic glycinin G1

Abstract

The acidic polypeptide chain of soybean glycinin G1 was identified as one of the several IgE-binding proteins by immuno-blotting with pooled sera from soy-allergic individuals. The region of IgE-binding within glycinin G1 was narrowed by in situ digestion with endoproteinase GluC followed by immuno-blotting of peptide products. This procedure resulted in a single IgE-binding fragment of approximately 15 kDa corresponding to residues 192 to 306 of the acidic chain of glycinin G1.