Spoligotyping of mycobacterium tuberculosis complex isolates by use of ligation-based amplification and melting curve analysis

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Abstract

We report here a ligation-based spoligotyping that can identify unamplified spacers in membrane-based spoligotyping due to asymmetric insertion of IS6110 in the direct repeat locus. Our typing yielded 84.4% (411/487) concordance with traditional typing and 100% (487/487) accuracy when confirmed by DNA sequencing.

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Zeng, X., Li, H., Zheng, R., Kurepina, N., Kreiswirth, B. N., Zhao, X., … Lia, Q. (2016). Spoligotyping of mycobacterium tuberculosis complex isolates by use of ligation-based amplification and melting curve analysis. Journal of Clinical Microbiology, 54(9), 2384–2387. https://doi.org/10.1128/JCM.00857-16

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