Growth of various strains of sulfate-reducing bacteria of human large intestine

Abstract

New strains of sulfate-reducing bacteria were obtained from human intestine. These bacteria are vibrio-like (strains SRB Vib-1, SRB Vib-2, SRB Vib-3) and short rod-like (strains SRB Rod-4 and SRB Rod-5) forms. Growth of these strains of bacteria, usa ge of sulfates by bacteria and their production of hydrogen sulfide were studied. The strains of sulfate-reducing bacteria SRB Vib-1, SRB Vib-2, SRB Vib-3 more intensively accu- mulate biomass compared with strains SRB Rod-4 і SRB Rod-5. The SRB Vib-1 and SRB Vib-2 strains grow most intensively. Accumulation of bacterial biomass (3.8 g/l) is the highest on the third day of cultivation, and after that a stationary growth phase be- gan. The SRB Vib-3 strain of bacteria accumulates 2.89 gram per liter of biomass on the third day of bacteria cultivation. Maximal biomass of SRB Rod-4 and SRB Rod-5 bacte- ria strains ranged from 2.59 to 3.25 gram per liter on the eighth day of cultivation. The obtained sulfate-reducing bacteria intensively use sulfate ions and produce hydrogen sulfide. The SRB Vib-1, SRB Vib-2, SRB Vib-3 strains produce from 2.99 mM to 3.12 mM of hydrogen sulfide. The rod-shaped strains of sulfate-reducing bacteria use sulfate ions and produce hydrogen sulfide less intensively in the presence of sulfates.