Bioremediation of petroleum hydrocarbon by using Pseudomonas species isolated from petroleum contaminated soil

Abstract

A newly isolated strain Pseudomonas fluorescens (Accession number KF 279042.1) have potential in diesel degradation and can be recommended for bioremediation of sites that are contaminated with diesel. This bacterium was characterized on the basis of microbiological, biochemical and molecular analysis. Bacterial growth optimization was studied based on carbon source, nitrogen source, pH and temperature. The strain was selected based on its ability to show growth in medium containing diesel. In addition, optimum temperature and pH for increased growth by the isolate were found to be 37°C and pH 8.0 indicating the maximum utilization of diesel. At the same time, production of protease and urease enzymes during the utilization of diesel was also assayed following the standard procedures.