Optimización de hidrólisis enzimática de la fracción globular de sangre bovina por metodología de superficie respuesta y evaluación de sus propiedades antioxidantes

Abstract

In this paper the reaction of enzymatic hydrolysis of the erythrocyte fraction of bovine blood was optimized through the response surface methodology. The effects of substrate concentration (S0:8-12 %), enzyme/substrate ratio (E0/S0:4-12 g/100 g protein) and pH (8-10), on degree of hydrolysis (DH) working to constant temperature of 55° C in a batch reactor, by 2 h were determined. The optimization of the statistical model obtained gave a maximum DH of 38.08 %, with pH 8.16; S0:6.6 and E0/S0:14.7, which was experimentally proved giving a DH of 36.25 ± 0.3 with negative bias of 5 %. At the optimal conditions samples were taken at three hydrolysis times to evaluate the antioxidant capacity, which was analyzed with ORAC, ABTS and FRAP methods. Greater antioxidant capacity was obtained at long hydrolysis time. After in vitro digestion it was confirmed that the antioxidant capacity of hydrolyzate remained.